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二肽基肽酶IV 检测试剂盒;原装进口,现货促销!

二肽基肽酶IV  检测试剂盒;原装进口,现货促销!
货号BML-AK499
DPPIV drug discovery kit    Enzo lifescience BML-AK499;二肽基肽酶 4 /二肽基肽酶IV  检测试剂盒,原装进口,现货供应!
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, providing enough material to perform at least 96 assays. DPPIV (DPP4, CD26) is a member of the class of proteases known as prolyl peptidases, which cleave proteins after proline residues and is thought to play roles in diabetes, cancer, and autoimmune diseases, making it a target for drug discovery.

The kit contains both a chromogenic substrate (H-Gly-Pro-pNA; Km=114 µM) and a fluorogenic substrate (H-Gly-Pro-AMC; Km=50 µM).

Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm. The kit is useful to screen inhibitors of DPPIV, a potential therapeutic target. A DPPIV inhibitor, P32/98 (KI=130 nM12), is included for use as a control. Other DPP enzymes are available for specificity profiling.
二肽基肽酶IV  检测试剂盒;原装进口,现货促销!
Product Specification
Alternative Name:
CD26 drug discovery kit, Dipeptidyl Peptidase IV drug discovery kit, DPP4 drug discovery kit
 
Long Term Storage:
-80°C
 
Kit/Set Contains:
DPPIV Enzyme (Human, recombinant) (Prod. No. BML-SE434-9090) (35 mU (40.4µl); 0.867 mU/µl. On U=1µmole/min@37°C, 100µM H-Gly-Pro-pNA. Purity ≥95% (SDS-Page)) Storage: -70°C; avoid freeze thaw cycles
pNA substrate (Prod. No. BML-PI188-9090) (150µl; 10mM in DMSO) Storage: -70°C
Calibration standard (p-nitroaniline) (Prod. No. BML-KI106) (1ml; 50µM in assay buffer) Storage: -70°C
AMC Substrate (Prod. No. BML-P189-9090) (150µl; 0.5mM in DMSO) Storage: -70°C
 
Calibration standard (7-Amino-4-methylcoumarin) (Prod. No. BML-KI107) (1ml; 40µM in assay buffer) Storage: -70°C)
Inhibitor (Prod. No. BML-PI142-9090) (20µl; 1mM in DMSO) Storage: -70°C
Assay Buffer (50mM TRIS, pH 7.5) (Prod. No. BML-KI342) (20ml; liquid in screw-cap plastic bottle) Storage: -20 or -70°C
½-volume clear microplate (Prod. No. BML-K101)Storage: Room temperature
二肽基肽酶IV  检测试剂盒;原装进口,现货促销!

相关文献:
Product Literature References
16, 17-Dihydro-17b-hydroxy isomitraphylline alkaloid as an inhibitor of DPP-IV, and its effect on incretin hormone and β-cell proliferation in diabetic rat: A. Shukla, et al.; Eur. J. Pharm. 47, 512 (2012), Application(s): Measurement of inhibition of DPPIV by DHIM, Abstract;
Discovery and preclinical profile of Saxagliptin (BMS-477118): a highly potent, long-acting, orally active dipeptidyl peptidase IV inhibitor for the treatment of type 2 diabetes: D. J. Augeri et al.; J. Med. Chem. 48, 5025 (2005), Abstract;
CD26/dipeptidyl peptidase IV and its role in cancer: B. et al.; Histol. Histopathol. 19, 1345 (2004), Abstract;
CD26/dipeptidyl peptidase IV: a regulator of immune function and a potential molecular target for therapy: U. Aytac et al.; Curr. Drug. Targets Immune Endocr. Metabol. Disord. 4, 11 (2004), Abstract;
Dipeptidyl peptidase IV inhibitors for the treatment of diabetes: A. E. Weber et al.; J. Med. Chem. 47, 4135 (2004),Abstract;
N-linked glycosylation of dipeptidyl peptidase IV (CD26): effects on enzyme activity, homodimer formation, and adenosine deaminase binding: K. Aertgeerts et al.; Protein Sci. 13, 145 (2004), Abstract;
Dipeptidyl peptidase IV (CD26) activity in the hematopoietic system: differences between the membrane-anchored and the released enzyme activity: D. A. Pereira et al.; Braz. J. Med. Biol. Res. 36, 567 (2003), Abstract;
Dipeptidyl peptidase IV inhibitor treatment stimulates beta-cell survival and islet neogenesis in streptozotocin-induced diabetic rats: J. A. Pospisilik et al.; Diabetes 52, 741 (2003), Abstract;
Prolyl peptidases: a serine protease subfamily with high potential for drug discovery: J. S. Rosenblum et al.; Curr. Opin. Chem. Biol. 7, 496 (2003), Abstract;
Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV: R. Thoma et al.; Structure 11, 947 (2003), Abstract;
On the regulatory role of dipeptidyl peptidase IV (=CD=adenosine deaminase complexing protein) on adenosine deaminase activity: I. Ben-Shooshan; Biochim. Biophys. Acta. 1587, 21 (2002), Abstract;
Human serum dipeptidyl peptidase IV (DPPIV) and its unique properties: H. Shibuya-Saruta; J. Clin. Lab. Anal. 10, 435 (1996), Abstract;
Activity of dipeptidyl peptidase IV and post-proline cleaving enzyme in sera from osteoporotic patients: H. Gotoh et al.; Clin. Chem. 34, 2499 (1988), Abstract;
 二肽基肽酶IV  检测试剂盒;原装进口,现货促销!
========================================================
二肽基肽酶(dipeptidyl peptidase ,DPP)
二肽基肽酶(dipeptidyl peptidase DPP)是蛋白酶家族中的脯基肽酶成员,其可剪切含脯氨酸的蛋白,其中DPPIV (DPP4, CD26),是一种丝氨酸二肽基肽酶,其剪切靶多肽(细胞趋化因子如CXCL11和肽类激素如GLP-1N端的X-Ala or X-ProCD26是一种高度保守、分布于多种细胞的,具有跨膜域和很短的胞质域,但常被剪切和释放作为可溶性环化4型,常形成自身二聚体或与FAP形成异二聚体。DPPⅣ的天然底物多种多样,在体内执行多种重要功能。它还是细胞膜受体和共刺激分子,从而参与机体的免疫调节、细胞移行、细胞黏附和细胞调亡过程,故与多种疾病的发病有关。各种DPP-IV抑制剂对II型糖尿病、自身免疫性疾病及细胞增殖性疾病(肿瘤)的治疗具有重要意义。DPP-4是重要的药物开发靶点,其中DPP-IV抑制剂Januvia磷酸西他列汀Sitagliptin  phosphate已作为抗糖尿病药物上市。
DPP相关产品:(biomol
Catalog #
Name
Category
size
AK-499  
DPPIV Drug Discovery Kit
 DPP  Assay Systems
96T
SE-527  
DPP8 (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-528  
DPP9 (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-564  
DPPII/DPP7 (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-529  
DPPIII (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-434  
DPPIV (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-409  
FAP (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
SE-545  
POP/PREP (human, recombinant)
 DPP Enzymes and Assay Systems
• Prolyl Peptidase Enzymes and Assay Systems
10 µg
P188-0025
GP-pNA, Chromogenic Substrate   
DPP 底物
25mg
P189-0005 
GP-AMC, Fluorogenic Substrate 2
DPP 底物
5mg
SA451-0100 
Anti-DPPIV
DDP抗体
100 µg
SA-472
Anti-FAPα
DDP抗体
100 µg
SE479-0100 
Cystatin C
DPP Inhibitors抑制剂
100 µg
EI300-0001 
EI300-0005 
GM6001
DPP Inhibitors抑制剂
1 mg
5 mg
PI142-0010 
PI142-0050 
P32/98
DPP Inhibitors抑制剂
10 mg
50 mg
PI112-0005 
PI112-0025 
Z-Prolyl-prolinal
DPP Inhibitors抑制剂
5 mg
25 mg
EI106-0010 
EI106-0050 
Baicalein
DPP Inhibitors抑制剂
10 mg
50 mg
 二肽基肽酶IV  检测试剂盒;原装进口,现货促销!二肽基肽酶IV 检测试剂盒;原装进口,现货促销!
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